War on clams has been declared at Lake Tahoe, a large freshwater lake in the Sierra Nevada Mountains of the United States. Scuba divers have been enrolled in a 400,000 USD project aiming to completely rid the lake of all Asian clams. The anti-clam endeavour is scheduled to begin in mid-March and is a combined effort by the governments of Nevada and California.

The Asian clam (Corbicula fluminea) is native to Asia and parts of Africa where it inhabits streams, canals and lakes. In this part of the world it is a natural part of the ecosystems and is even known as the prosperity clam or good luck clam. The Asian clam was introduced to North America in the 1920s by Asian immigrants for whom it was an appreciated source of food. It would however take until 2001 before the first specimens were encountered in Lake Tahoe. Since the first finding, the clam has been collected from numerous locations Tahoe’s southeast shore and authorities now fear that it will pave the way for even more dangerous invasive species such as the Ukrainian Quagga mussel (Dreissena rostriformis bugensis) and the Russian Zebra mussel (Dreissena polymorpha).

”We’re concerned they could create a positive settlement situation for the quagga mussels,” says Steve Chilton, aquatic invasive species coordinator for the U.S. Fish and Wildlife Service. ”We’re basically looking at all avenues through which the quagga mussel could get into the lake and eliminate that risk factor as much as possible.”

Steps so far have primarily focused on mandatory boat inspections to ensure no mussels are attached to them when they launch into the lake, but scuba divers participating in the new anti-clam project will actually be removing Asian clams from Lake Tahoe’s southeast shore. Starting in mid-March, divers will place plastic sheets, so called bottom barriers, over selected clam beds in order to deprive the clams of oxygen and nutrients. Divers will also carry out ”diver-assisted suction”, e.g. manually vacuum clams off the bottom of Tahoe.

”This needs to be done. We have to get our hands around the Asian clam problem,” Tahoe Regional Planning Agency spokesman Dennis Oliver told the Reno Gazette-Journal. ”We need to find out what works and what works best. Once we know that, we can develop a program.”

Invasive mussel species are known to form huge populations in environments where they lack natural predators and can for instance clog water intakes, attach themselves to boats and docks, and litter sandy beaches.

North America is not the only continent with an Asian mussel problem; Corbicula fluminea has begun to spread throughout Europe as well. It was found in the Rhine as early in the 1980s and then gradually found its way into the Danube through the Rhine-Maine-Danube Canal. In 1998 the first specimens were found in the Elbe and the species is now also present in the rivers of Portugal.

The U.S. Bureau of Reclamation is now carrying out tests in hope of finding out if bacteria can aid them in their struggle against invasive mussel species that are threatening to spread across the West’s waterways.

During the summer of 2008, a preliminary test was executed at Davis Dam on the Colorado River at Laughlin in Nevada. In this dam, Quagga mussels (Dreissena rostriformis bugensis) were exposed to dead bacteria of the Pseudomonas fluorescens species, a non-infectious bacterium that is commonly found in water, soil and food.

Quagga Mussels

During the first test the mussels where exposed to bacteria in jars, but the next test will take place in 10-20 gallon aquariums to in order to more accurately mimic real dam conditions. Water will flow through the aquariums, but will not be released back into the river – it will instead be disposed of through an evaporation pond. A third experiment is also planned, where bacteria will be released in a domestic water intake line which is currently encrusted with a 2-3 inches thick layer of mussels (approximately 5-7.5 cm).

“We are always looking for new, more effective techniques for managing mussels, and this one looks very safe and very promising,” says Reclamation scientist Fred Nibling. “We’ll have a series of tests where we’re going to be testing off-line, off the river, so we can have the data to where we can apply for the permits to test elsewhere.

If the initial testing proves to be successful, the Bureau of Reclamation hopes to have a larger scale test approved by the Environmental Protection Agency.

The U.S. Bureau of Reclamation got the idea to use Pseudomonas fluorescens from Daniel Molloy, a researcher at the New York State Museum who discovered that both zebra and quagga mussels died if they ingest the bacterium. He confirmed the effect in 1998 and the method was patented by the museum. Eventually, the Californian firm Marrone Organic Innovations was awarded a National Science Foundation grant to commercialize the technology.

According to Molloy’s research, a mussel needs to ingest a high density of a strain of the bacteria in order for the bacteria to be lethal. If the density is high enough, a toxin inside the bacterium cell will efficiently devastate the digestive tract of the animal.

One advantage with Pseudomonas fluorescens compared to conventional anti-mussel treatments like chlorine is that mussels recognize chlorine as dangerous and close their feeding valves to keep the chemical out. They do however happily devour Pseudomonas fluorescens. Another important aspect is that research has found that Pseudomonas fluorescens does not kill fish or shellfish.

If large scale testing also proves successful, the Bureau of Reclamation say they wish to meet with municipal public works and water authority officials before the bacterium is put into general use. “We want to make sure they’re very comfortable and they have a chance to ask questions,” says Nibbling.

Zebra mussels