The ND 41 is a hybrid between a female Blue tilapia (Oreochromis aureus) of the ND-4 Tilapia family line and a male Blue tilapia (Oreochromis aureus) of the ND-1 Tilapia family line. The ND-4 and the ND-1 are have been created by man through continuous selective breeding and crossing other types of Oreochromis aureus will not produce the same result. 

ND41 tilapia comes with four main advantages:

• When a female ND-4 and a male ND-1 of the species Oreochromis aureus breeds, the amount of male offspring in any spawning cycle will be around 98 percent. (The 2 percent female offspring is caused by penetration of foreign fish; without foreign genetic material the batch will contain 100% males.) There will therefore be no need to use hormones to achieve sex reversals in the offspring.
    
• ND 41 is a fast grower with a growth rate that actually exceeds that of the famous Oreochromis nilotica in the temperature range 22-25 °C (72-77 °F). The growth rate will however be lower than that of Oreochromis nilotica in the temperature range 29-31 °C (84-88 °F).
  
  
• The ND41 is calmer than Oreochromis niloticus, a big plus during sorting, sampling, selective harvesting and other forms of handling. Fatalities brought on by handling are normally significantly less frequent in docile fish since they are more cooperative. 
  
  
• ND-4 and ND-1 tilapia is capable of producing offspring at low temperatures and will engage in mating behaviour at 18 °C (65 °F). When the water temperature reaches 20 °C (68 °F), spawning will be apparent. This is highly beneficial when tilapia is breed outside the tropics and can also be of interest in tropical regions that experience a few months of reduced temperature each year.
  
  ND21 tilapia has the same resilience to Streptococcus infection as pure line Oreochromis niloticus.