Hi--I have treated the water and prepared the tank. I have never done fishless cycling and didn't understand cycling at all (still don't) completely before I found this site. I have the API master test kit and just did a lot of worrying and partial water changes forever til my nitrites disappeared. I still have ph problems at times and don't know why. I have a 60 gal , 40 gal turtle, 2 10's, and 1 5gal plus the new column tank. I am thinking of just cycling it with fish as usual. Also, could I put just one or two fairly large fish in it plus a pleco or flying foxes? It has monster aeration! Thanks for any help! Shirley