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View Full Version : Yeast...a brewers perspective.


BrandonBCA
02-17-2011, 07:07 PM
From reading this forum, I gather most use only sugar+yeast in their recipes for DIY CO2. While this can be effective in the short term, it will waste sugar, and produce a far less stable cycle of CO2 generation(sharp peaks). A little understanding of the processes going on in your fermenters will help get the most from your yeast and more constant production of CO2.

First off the reaction we're most interested in is:

Sugar+phosphate+pyruvate=ethanol+CO2+ATP

The goal of a good recipe should attempt to maximize the utilization of the sugar to the maximum levels of alcohol tolerated by the yeast, which sugar water will not provide. How you provide these nutrients is optional, and there are numerous possiblities so you can just use what you have on hand. Some of these options would be fish food, molasses, a vitamin rich juice, multivitamins or a specific yeast nutrient. This list is not exhaustive.

Yeast cells contain all the nutrients needed for yeast, but due to their thick cell membranes they are not readily available. If you wish to make your own yeast nutrient you will need to lyse the yeasts cell walls. The way to accomplish this is to heat kill the yeast in an acid(lemon juice works well) solution, then freeze it(in a small ice cube tray for easy dosage) to completely pop the membranes. The heat kill is similar to a pasteurization process, whereby you boil the yeast+acid at a rolling boil for ~15 minutes.

Of minor note, as the fermentation progresses the yeasts ability to process certain forms of phosphate and nitrogen is lessened, so for commercial yeast nutrients ammonium salts of phosphate(specifically DAP) are added in addition to the traditional yeast nutrient.

Rehydration yeast nutrients(such as Go-Ferm) are largely unnecessary for our purposes, but a fermentation extending yeast nutrient(such as Fermaid K) are reasonably priced, long lasting and necessary for a complete fermentation. If you live somewhere it is available marmite, vegemite and other lysed yeast extracts will work well as a nutrient. This is not the same as the nutritional yeast or brewers yeast sold in drug stores.

Different yeasts have different characteristics in their fermentations. The four characteristics we are most interested in are:

1)stability of production
2)total alcohol production
3)flocculance-it's the gunk that gets in the lines
4)operating temperature

Yeasts are broadly categorized into beer,baker's, wine, and fuel yeast strands. I will not discuss fuel yeasts since they are not readily available to the buying public.

Beer yeasts are high flocculants with low alcohol tolerance, fast fermentations, and high operating temperatures. None of these characteristics make them ideal for our purposes.

Baker's yeasts are high flocculants with middling alcohol tolerance(~13%), moderate fermentation periods and moderate operating temperatures. They are adequate for the CO2 generators, especially if low flocculant producing foods are fed. The amount of sugar in solution that would get the best yield is to a specific gravity of ~1.1, which would be about 5 cups/gallon or 1 1/4 cups/liter.

Wine yeasts are low flocculants with the highest alcohol tolerance among readily available yeasts, have the lowest operating temperatures(Around 55F) and produce the slowest, most easily predictable CO2 yields. All of these characteristics make them the ideal yeast to use in our ferment generators, especially for those of us who like to keep the thermostat down this time of year.

Finally, shaking the bottle is neither necessary nor harmful. Commercial beer fermentation often uses a continuous fermentation process, in which the beer is continually stirred for an even flavor/to speed up the reaction. The only way I can figure this bit of nonsense got injected into the DIY culture is through a misunderstanding of directions on breadmakers, where they say not to mix the yeast in a timed-bake batch. This is purely to keep the yeast from hydrating early, just as you wouldn't want your coffee grounds wet in a delay brew coffee situation.

BrandonBCA
02-17-2011, 08:13 PM
Furthermore, yeast goes through 3 stages in it's lifespan, and all 3 are important to understand.

First is reproduction. This is when you first rehydrate the yeast in an oxygen rich environment. This stage produces some CO2, though I am not sure how much and capturing the CO2/maintaining this stage would be difficult for an aquarium DIY setup.

The second, and by far the most important to us is fermentation. Specifically we are interested in primary fermentation,considered an aerobic fermentation. It takes place best at a pH of between 5.3 and 5.5, so for the most vigorous setups with more maintenance a pH buffer for this range may be added. These could have the added benefit of containing phosphate, which the yeast will utilize. However, many DIYers wish to slow down the reaction for longevity, which is the logic behind adding baking soda. Generally, no buffers are necessary but your water chemistry should help you decide for yourself.

The final stage is sedimentation. It is essentially a dormancy period for the yeast. This occurs when either a)no more sugar is present b)alcohol content exceeds yeast tolerance or c)nutrients necessary in the pathway for pyrovate production or there is a lack of available phosphate. This is the stage at which we add nutrients or change the solution. After experimenting through a few bottles you should be able to tell which to do. If sugar is present and was added in an amount such that it would exhaust when the alcohol level was at the yeasts tolerance threshold, the yeast should reactivate within an hour and the solution will fog back up.

Finally, bottle hygiene is important. Once a sugar solution is exhausted the yeast(the gunk on the bottom of the bottle) may be retained for the next batch, however it is best to transfer it to a new clean bottle. The old bottle should then be cleaned thoroughly. Rinsing thoroughly with hot water should suffice, though a rinse with a diluted bleach solution followed by a vinegar rinse and finally thorough water rinsing shouldn't hurt. Using a commercial de-chlorinater, however, can due to the presence of sulfite ions. A wine yeast would survive commercial de-chlorinater well and often sulfite is added to wine rehydration nutrients so wild yeasts which can ruin the flavor will be killed and replaced by the sulfite-tolerant yeast strains.

Lady Hobbs
02-17-2011, 08:23 PM
Some methods don't even require yeast at all. Baking soda and vinegar is another method mentioned in articles.

BrandonBCA
02-17-2011, 08:43 PM
True enough, though for the acid+carbonate reaction calcium carbonate(limestone or chalk) is a better source of CO2 since for the acetic acid/sodium bicarbonate reaction occurs in 2 stages

bicarbonate=CO3+water

Hydrogen ions+CO3=CO2+water

whereas CaCO3 only has
Hydrogen ions+CO3=CO2+water

thus you get more CO2 yield per dry pound of solute. These methods are better than yeast to produce controlled CO2, but can be more complicated to setup.

There are also methods using mycellium, compost, electrolysis, and combustion. Each has it's own drawbacks and benefits. If CO2 were hard to produce, it wouldn't be as much of a concern as it is. My goal in this write up is to help those using the yeast method, not to be an exhaustive DIY CO2 primer.